Analysis of inulin and fructans in Taraxacum officinale L. roots as the main inulin-containing component of antidiabetic herbal mixture

Herbs and their combinations due to the wide range of biologically active substances can influence on various links pathogenetic mechanism development diabetes mellitus its complications. One such is an antidiabetic herbal mixture with established hypoglycemic, hypolipidemic, antioxidant, hepatoprotective, pancreatoprotective activity in previous pharmacological study vivo that including inulin-containing component – Taraxacum officinale L. roots. Thus, aim this was determine quantitative content inulin fructans Quantity determined by difference between fructose as a product enzymatic hydrolysis D -fructose, constituent sucrose free taking into account empirical factor for conversion -fructose from inulin. Carbohydrates used calculation were separated gas chromatography-mass spectrometry after volatile derivatives aldononitrile acetate. According results, roots contain 436.29 mg/g Total spectrophotometric analysis acid 5-(hydroxymethyl)furfural. The results show 39.49% fructans. obtained are evidence plant should be included mixture, because presence causes hypolipidemic detoxification activity.